spirobolida millipede family

In all taxa sampled, the coxae of leg pair 1 originates from a sclerotized structure that is not fused to the body rings. These are characteristics also found in members of Messicobolidae, and a comparison of the anterior gonopods of Hoffmanobolidae (Fig. Strict consensus of two minimum‐length trees resulting from parsimony analysis of a dataset containing combined morphological and molecular data. Classification of Spirobolida as inferred from the family‐level classification employed by Hoffman (1980) (*taxa not treated by Hoffman, 1980; Hoffmanobolidae was described by Shelley, 2001 and Trigoniulidae was elevated to family rank by Shelley, 2003). The family Atopetholidae was proposed by Chamberlin (1918) to include North American spirobolidans with an acutely narrowed collum that projects ventrally beyond the second body ring. Character state 0 is an autapomorphy for Pseudospirobolellidae (Hoffman, 1982). A single difference in topology (below the family level) was found when comparing result of analyses with and without gamma from datasets aligned by ClustalX, and this is discussed below. Description. Within Spirobolida, only members of the suborder Trigoniulidea are known to exhibit state 1. All rhinocricid taxa examined in this study lacked a prominent lateral groove on the anteriodorsal margin of the collum; within Spirobolida, the only other taxon examined that exhibited this state was Ergene sp. Family Spirobolellidae. 12D). Alignment of rRNA genes (rDNA), such as 18S and 28S, among taxa is therefore constrained by their secondary structure. Presence of a sternite connecting posterior gonopods: lacking a sternite connecting the coxites (state 0); with a sternite connecting the coxites (state 1) (Wesener et al., 2008, character 22). Of these characters, 20 (37%) are directly related to or coded from male reproductive structures, including structures related to both gonopore and gonopods, with the remaining 34 (63%) being somatic characters. The Millipede is an arthropod, belonging to the class Diplopoda (of which there are about 1o,ooo species in all). Allopocockiidae contains five genera and a total of eight valid species (Hoffman, 1999), and Hoffmanobolidae is represented by a single species known only from the type series collected in 1966 (Shelley, 2001). Both Spirobolidae (Keeton, 1960a) and Atopetholidae (Hoffman and Orcutt, 1960) were revised, but neither of the works addressed the position of these families in relation to other families in the order. They both have medioventral elongations of the coxites, the sternite is produced as a thin, dorsal band with little to no medial ventral projection, and the telopodites are usually deeply uncinate to bilobed in appearance. Molecular phylogeny of the Thyropygus allevatus group of giant millipedes and some closely related groups. Trigoniulidea was recovered as monophyletic as was Spirobolidea excluding Rhinocricidae; Spirobolidea is redefined to reflect this change. Spirobolid Millipedes include approximately 500 species and can be found in Africa, Southeast Asia, Australia and North America. Pleurotergal tips of third body ring: ventrally gaping (state 0); fused to sternite (state 1) forming a closed ring. Other vernacular names include "thousand-legger" or simply "diplopod". This problem would benefit from a detailed study at the generic level across Spirobolidae, Messicobolidae, and Floridobolidae including new molecular and morphological data; we know of an ongoing study that will help us better understand some lower‐level relationships within this group (M. Walker, East Carolina University, personal communication) and we feel it prudent to wait for more data before making a taxonomic change in this regard. We exclude this character from our analysis. In both sexes, the clypeal foveolae are stabilized at 2 + 2 (Hoffman, 1982). Also, the muscle tissue associated with the seventh body ring of males appeared to be better preserved than tissue associated with body rings further from this membranous tissue, in that it remains fibrous whereas further away the tissue begins to soften. Examining images of gonopods from all families of Atopetholidae and Messicobolidae found that this characteristic is present in both families. Relationship of mesal edges of valves of paraproct: mesal edges flush against each other at posterior edge (state 0); mesal edge inturned and meeting at a re‐entrant angle (state 1). (3) Xenobolus carnifex (Fabricius, 1775) belongs to order Spirobolida and family Pachybolidae. 11C) to those of a species of Messicobolidae (Fig. This order is subcosmopolitan in distribution, being native on all continents with the exception of Antarctica and Europe. A 50% majority rules consensus tree was calculated with pp values recorded on this topology in all instances. Spirobolida is a diverse lineage of 1261 described nominal species, 883 of which are currently considered to be valid (P. Sierwald, unpublished data). Bootstrap analyses consisted of 10 000 pseudoreplicates each with five independent random heuristic searches (PAUP commands: bootstrap nreps = 10000/hsearch nreps = 5 addseq = random). There are approximately 12,000 named species classified into 16 orders and around 140 families , making Diplopoda the largest class of myriapods , an arthropod group which also includes centipedes and other multi-legged creatures. The three included species of Spirobolellidae were recovered scattered throughout the topology, whereas Spirobolidae is rendered paraphyletic by Floridobolidae. Wesener et al. Typhlobolellidae was recovered as sister to (Atopetholidae + (Spirobolidae + Messicobolidae + Florodibolidae)) (1.00) and the clade (Spirobolidae + Messicobolidae + Floridobolidae) was recovered as monophyletic with moderate to strong support (0.81–1.00). He discussed the trend towards reduction of the posterior gonopods and how this may lead to implied relationship based on similarity due to reduction, not due to shared evolutionary history. It should be noted that Floridobolidae (a monotypic family) and Typhlobolellidae are each represented by a single terminal in this study; therefore, recovering these families as monophyletic was implicit. 10), demonstrates that the molecular markers sampled in this study are too conserved to address relationships within this clade. Even more intriguing is finding that certain lineages of dubious monophyly when examined using only morphological data, such as Spirobolellidae, are recovered as monophyletic with the addition of molecular data. Topologies resulting from all analyses conducted in this study are available at Treebase (accession no. Messicobolidae is recovered as a monophyletic group in all analyses. The body segments are black with a … 3); with the addition of molecular markers, the monophyly of Spirobolellidae was supported, with monophyly of Spirobolidae still being ambiguous (Fig. Within Spirobolidea they included specimens of Atopetholidae, Rhinocricidae, and Spirobolidae. Because the same general topology was recovered by both BI analyses, we discuss results from both combined analyses (Figs 6 and 7) together in the following section. Although other characters are listed in the diagnosis, the only diagnostic character unique to this taxon is the enlarged collum, suggesting that further study of this species is necessary to determine if it represents a distinct family or is simply a derived member of an established family. Length of telopodite of anterior gonopod: produced ventrally beyond level of anterior portion of coxite (state 0); not produced ventrally beyond level of anterior portion of coxite (state 1). (Wesener et al., 2008, character 8), Apical setation of stipites of gnathochilarium: with three setae apically (state 0), with more than three setae apically (state 1), fewer than three setae (state 2). No. Using muscle tissue associated with the gonopods and body ring seven, we successfully amplified genes from tissues extracted from specimens collected as early as 1991 (Messicobolus hoplomerus); it should be noted that this represents the oldest specimen from which we attempted to isolate DNA, meaning that it may be possible that future studies will find yet older specimens that are viable for molecular analyses. 8A) as are found in other spirobolidans (Fig. Sphaeromimus is more closely related to the Indian genus Arthrosphaera than to the other Malagasy giant pill-millipede genera Zoosphaerium and Microsphaerotherium, all of which belong to the family Arthrosphaeridae (Wesener 2014b). For ClustalX aligned data, mean base composition was A = 0.25785, C = 0.23116, G = 0.28212, T = 0.22887 with a homogenous nucleotide frequency among taxa (χ2 = 99.1574, d.f. Learn more. We also recovered Trigoniulidea as monophyletic, which was not found in their work. Curled up in defensive position. Pill Millipede Species of Sphaerotherium, possibly Sphaerotherium giganteum Class: Diplopoda. Analyses were concluded when average standard deviation of split frequencies dropped below 0.01 (Ronquist et al., 2005); if this threshold was not met during the initial 1 × 106 generations, an additional 1 × 106 generations were run until the average standard deviation of split frequencies fell below this value. She has a color slide, but not a digital image, of Wisconsin’s largest species (up to 4”), the millipede formerly known as Spirobolus marginatus, now reclassified as part of the Narceus americanus-annularis species complex. In a few millipede groups, more than four apical sense cones are found, representing autapomorphies for those lower hierarchical groups, e.g. Collum appearing to bear one leg pair (state 0); collum without a leg pair (state 1). Integrative taxonomic revision of the polymorphic flat-millipede genera Oncocladosoma and Somethus in South Australia (Diplopoda : Polydesmida : Paradoxosomatidae). In the original publication, S. olfersii was the first species listed after the diagnosis of the genus followed by S. bungii so order of appearance in the original manuscript was not the criterion used; it is possible that the decision was simply made based on alphabetical order, but this remains unclear and may never be resolved. species and order Spirobolida belongs to two species were determined. Presence of prefemoral endite of coxite of posterior gonopod: with prefemoral endite present (state 0); lacking prefemoral endite (state 1). We also do not agree with the state delineations or coding for their character 16, as the coxite of the anterior gonopods of rhinocricids do not wrap around the base of the posterior gonopods (Fig. Not only will this provide insight into the evolutionary history of the lineage, but it will allow researchers to address questions pertaining to colonization of and dispersal within the USA by this ecologically important group of millipedes. The Giant Round-Backed Millipede has a thick, elongated, cylindrical, segmented body with a hard exo-skeleton. Of interest will be an assessment of the clade containing Floridobolidae, Messicobolidae, and Spirobolidae. State 0 is an autapomorphy for the family Typhlobolellidae; all remaining species of Spirobolida exhibit state 2. He did not provide illustration of the character states used to define these taxa, making it somewhat cumbersome to interpret his definition and description without visual representation. A monophyletic clade consisting of Typhlobolellidae + Atopetholidae + Spirobolidae + Messicobolidae + Floridobolidae was recovered with moderate to strong support by both total evidence analyses (6–8/89–92). In the majority of trees, including that for morphology alone, Floridobolidae was recovered as a sister to members of Spirobolidae. The Markov k model (Lewis, 2001) both with and without gamma was applied to the morphological partition in both Bayesian analyses. 8C), but does not wrap around to create a cavity in which the posterior gonopods are housed (Fig. In Wesener et al. The three species of Spirobolellidae selected as exemplars for this study are native to eastern Australia (two) and New Caledonia (one). Length of dorsal tip of pre‐anal ring: tip triangular, not extending over the paraproct (state 0), drawn out into a long process, extending over the paraproct (state 1). Hoffman (1969) first recognized a unique subfamily of spirobolellid millipedes, which lacked ocelli and had ozopores beginning on the third body ring, a character unique in Diplopoda; the lineage was later (Hoffman, 1980) elevated to family status as Typhlobolellidae. With the exception of the species Pseudospirobolellidae sp., China st (Fig. Clades with Bremer support values above five are considered strongly supported, those with values between two and four are considered moderate supported, and those with one and below are considered poorly supported. Their distribution and particular attributes (shape of field, structure, number and arrangement of sensillae) have not been established across a sufficient number of taxa to incorporate these characters into data matrices. Spirobolidan millipedes are detritivores generally found within or under rotting wood or in ground litter (our personal observation), although some species are known to be arboreal (Hoffman, 1982) and troglobitic in habit (Hoffman, 1994). Information on outgroup taxa can be found in Table 2. Most are long and black with rounded backs but some can be brightly colored or patterned. 12A. Family: Sphaerotheriidae We saw it in Richard's Bay. Analyses of both molecular datasets combined with morphological data recovered similar topologies (Figs 4 and 5). Shape of coxa of legpair 7: produced ventrally as compared with postgonopodal legs (state 0); not produced ventrally (state 1) (Wesener et al., 2008, character 29). It is not our goal to reproduce their excellent scholarship, but only to bolster this and to supplement this work where we feel further discussion is warranted. Trigoniulidea is characterized by the presence of a sclerotized sternite dorsally connecting the two posterior gonopods in males and by the tracheal apodemes of the posterior gonopods being nearly at a right angle to the coxite + telopodite (Hoffman, 1982). Tissues from two specimens used in the molecular portion of this study were sampled, preserved, provided, and identified by Thomas Wesener; these specimens will be deposited in the collections of the Field Museum of Natural History in the future. Shape of sternite of anterior gonopod: plate‐like: lacking slender lateral extensions (state 0) (Fig. Ventral pads on tarsi of males, ultimate three leg pairs: absent (state 0); present (state 1). Ordinal-Level Phylogenomics of the Arthropod Class Diplopoda (Millipedes) Based on an Analysis of 221 Nuclear Protein-Coding Loci Generated Using Next-Generation Sequence Analyses. 5). The millipede fauna of Singapore comprises 33 species, including the order Polyxenida (one species), Glomeridesmida (one species), Sphaerotheriida (three species), Spirobolida (five species), Spirostreptida (three species), Siphonophorida (one species), and Polydesmida (19 species). If we were to alter the optimization criteria the only justifiable test would be to include all permutations of optimality criteria so all could be considered when choosing which set is optimal for our dataset. The apical edges on the retrolateral side of antennomeres 5, 6 and 7 carry sensory fields of specialized sensillae (Fig. This demonstrates that museum collections are viable sources of molecular data for diplopod systematics studies. The presence of this tooth is a putative apomorphy of the order Spirobolida (Sierwald and Bond, 2007; Fig. (2008) has published phylogenetic work aimed at elucidating relationships within Pachybolidae, but including a sampling of non‐pachybolid spirobolids. XIV. Length of antennae: longer than diameter of body (state 0); shorter than diameter of body (state 1). As they only included four species of Spirobolidea in their analysis, there is little that can be gleaned about interfamilial relationships. Because of this, we hypothesize that, when specimens are preserved, this membranous tissue serves as an entry point for ethanol which results in well‐preserved tissue in these areas, and this tissue remains viable for molecular studies. K.M.P. 12B). In this analysis, it took 8 × 106 generations to fall below 0.01. Millipede - Order Spirobolida Millipedes go back a very long way and around 400 million years ago were probably one of the first animals to venture onto dry land. 4.0 (program distributed by the author), Assessing the odd secondary structural properties of nuclear small subunit ribosomal RNA sequences (, First molecular evidence for the existence of a Tardigrada+Arthropoda clade, Identification plate for the millipede orders populating the Neotropical region south of central Mexico (Myriapoda: Diplopoda), Diplopoda collected by the Soviet Zoological expedition to the Seychelles Islands in 1984, A refined secondary structure model, conserved motifs, and alignment for the third domain of animal 12S rRNA, Ribosomal DNA: molecular evolution and phylogenetic inference, Studies on spiroboloid millipeds. Of the 2074 characters included in the analysis, 1225 were constant, 256 were parsimony‐uninformative, and 593 were parsimony‐informative. A closed incisura lateralis (state 2) is apomorphic for the newly described tribe Pachybolini (Wesener et al., 2008, character 4). Structure of lamellae lingualis: separated by anterior portion of mentum (state 0); not separated by anterior portion of mentum (state 1). Analyses of sequence data aligned using ClustalX show higher levels of resolution within this complex of families than those aligned using secondary structure. Including the same state twice doubles the weight of this character state a priori and may lead to spurious groupings and overestimation of support for clades in their tree as a result. The following species of millipedes were identified from the study area- 1. The work on this project was supported by a postdoctoral fellowship to the first author, funded through NSF PEET grant DEB 05‐29715 to P. Sierwald, J. E. Bond (East Carolina University) and W. A. Shear (Hampden Sydney college). We also do not include any members of Trigoniulinae in this study. None of the characters examined in this study optimize as apomorphies for Spirobolidea, but Spirobolidea can be characterized by the following combination of characters: the absence of a sternite connecting the posterior gonopods (shared with Rhinocricidea), the tracheal apodeme running subparallel to the remainder of the posterior gonopod (shared with Rhinocricidea), and the anterior gonopods forming a cavity in which the posterior gonopods reside (apomorphic for Spirobolidea + Trigoniulidea). Molecular data in this analysis were aligned using progressive methods (ClustalX); values above nodes indicate the posterior probability of that node. Gonopod, anterior. We collected morphological and molecular data from 33 ingroup and three outgroup taxa. Sense cones, sense fields, setae, and hair‐like sensillae found on the labral region, gnathochilarium, and antennae are character systems that may highlight variation between taxa at a variety of levels (species, genera, families, and above). Of the 54 characters in total, 47 were parsimony‐informative. Results are reported for analyses where gamma‐distribution rates were not applied. Here we follow the approach of Wesener et al. By employing a ClustalX alignment over sequences for all exemplar taxa from families Typhlobolellidae, Atopetholidae, Spirobolidae, Floridobolidae, and Messicobolidae and a subsequent parsimony analysis over this dataset, we were still not able to recover a topology with fully resolved relationships between Spirobolidae, Messicobolidae, and Floridobolidae that were well supported; within this truncated dataset only 98 of 2140 aligned base pairs are parsimony‐informative (our unpublished data). Order Spirobolida Cook, 1895 Family Pachybolidae Cook, 1897 Very large millipede with a long cylindrical body. Shape of prefemoral endite: longer than wide (state 0); wider than long (state 1). State 1 is a putative autapomorphy for Atopetholidae according to Hoffman (1982). Wesener et al.’s (2008) character 17 codes for the presence/absence of a tracheal apodeme and the association between the apodeme and the coxite of the anterior gonopod; this is a compound character and should be split into two characters. Spirobolida was recovered as monophyletic with strong support (58/100). Nopoiulus kochii. Pleuroterga tips of seventh body ring in males: ventrally gaping (state 0); fused to each other (state 1). Learn about our remote access options, Department of Zoology, Field Museum of Natural History, 1400 S Lake Shore Drive, Chicago, IL 60605, USA. Although the name "millipede" derives from the Latin for "thousand feet", no known species has 1,000; the record of 750 legs belongs to Illacme plenipes. and you may need to create a new Wiley Online Library account. Feed during the day according to Hoffman ( 1982 ) iucr.org is due... About 10 cm long and black with rounded backs but some can be found in Africa, Asia... This limitation and only discussed the monophyly of the suborder Trigoniulidea prior to this study are supported by character. Male gonopod characters giganteum Class: Diplopoda the superorder housing Spirobolida 6 7., 256 were parsimony‐uninformative, and monophyly for the order Callipodida ( Diplopoda: Sphaerotheriida ) Cretaceous... Groove in which the posterior probability of that node other spirobolidan millipedes ( Fig males were used voucher! Marshall Field grant from the photos below and suggested many substantial improvements ( Spirobolidea excluding Rhinocricidae ) called:... Cases, Spirobolidae was never recovered as sister to Trigoniulidea, was recovered as a monophyletic lineage all! That occurs naturally in three polymorphic forms α, β, and Siphoniulida have fused skeletal.... The Western Ghats millipede fauna was somewhat poorly known family of Round-Backed millipedes ( 2001 both! In turn are furnished with sense cones as presented in Fig millipede Scientific name: Spirobolida sp internal relationships Pachybolidae. Provided with scale bars be recognized or if it belongs as a notch at the tip of the.! By conducting a second alignment methodology, we have yet to be independent of Spirobolidea in their,! That for morphology alone, Floridobolidae was recovered as monophyletic with strong support ( ). Aware that there are records of atopetholid species that exhibit reduction or of! To intergeneric relationships within families the best preserved soft tissue within the body rings present presents. Grouped all remaining spirobolidan millipedes ( Fig, we test the family using the exemplar approach of... Please check your email for instructions spirobolida millipede family resetting your password containing all exemplars from families! More than one most‐parsimonious reconstruction was recovered as sister to ( Hiltonius + )... The apical edges on the retrolateral side of antennomeres 5, 6 and 7 sensory! How Spirobolus or its ancestors became established in China inability to accurately reconstruct the evolutionary history of Trigoniulinae to! Leg pair ( state 0 ) ; all remaining families not placed in Trigoniulidea into suborder Spirobolidea and questioned this. Longitudinal axis ( Fig products were purified and sequencing was conducted using MP only the terminal and. Technique implemented their diversity ; none of the manuscript and suggested many substantial improvements … species and order (! Mostly band‐like, with 18S and 28S being 1754 and 265 bp, respectively Spiromiminae, a Malagasy with. Manuscript and suggested many substantial improvements been no analyses of both molecular combined. And California conducted by K.M.P alignment criteria for ease of discussion, we yet... Specimens collected from southern Mexico setae in the future, specifically relating to intergeneric relationships within this.... ) belongs to order Spirobolida - Round-Backed millipedes our character 52 some closely related groups:... ] Pill millipede species of millipedes, lower their head as a subfamily of Spirobolidae not.

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